10000U Penicillin 10mg/mL Streptomycin 200 mM L-Gln L-Glutamine–Penicillin–Streptomycin Solution
Short description
10,000U penicillin 10mg/ml streptomycin 200 mM L-gln L-Glutamine–Penicillin–Streptomycin solution (with 200 mM L-glutamine, 10,000 U penicillin and 10 mg steptomycin/mL in 0.9% NaCl, sterile-filtered, BioReagent) is a high-quality product suitable for mammalian cell culture. It is used to reduce or eliminate bacterial contamination in cell, especially primary cell, cultures. ModeG1146, L-Glutamine–Penicillin–Streptomycin solution, with 200 mM L-glutamine, 10, 000 U penicillin and 10 mg steptomycin/mL in 0.9% NaCl, 0.1 Œºm filtered, BioReagent, is a reliable and effective option for cell culture. Known as Pen/Strep or Penicillin/Streptomycin, it provides optimal conditions for cell growth without compromising the purity and quality of the culture.
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E-commerce Store Punjab
India
2025 years
Description
10,000U Penicillin 10mg/mL Streptomycin 200 mM L-Gln L-Glutamine–Penicillin–Streptomycin Solution
The 10,000U Penicillin 10mg/mL Streptomycin 200 mM L-Gln L-Glutamine–Penicillin–Streptomycin Solution is a high-quality product specifically designed for mammalian cell culture. This solution is used to effectively reduce or eliminate bacterial contamination in cell cultures, particularly primary cell cultures. With its reliable and effective composition, it provides optimal conditions for cell growth while ensuring the purity and quality of the culture.
Features:
- High-quality cell culture solution
- Contains 200 mM L-glutamine
- 10,000 U of penicillin per mL
- 10 mg of streptomycin per mL
- Sterile-filtered to ensure purity
- BioReagent grade
Composition:
The 10,000U Penicillin 10mg/mL Streptomycin 200 mM L-Gln L-Glutamine–Penicillin–Streptomycin Solution is composed of the following:
- 200 mM L-glutamine: L-glutamine is an essential amino acid that plays a crucial role in cell metabolism and growth. It is supplemented in the solution to provide the necessary nutrients for optimal cell growth.
- 10,000 U penicillin per mL: Penicillin is a widely used antibiotic that effectively inhibits the growth of Gram-positive bacteria, including some common contaminants in cell cultures such as Staphylococcus and Streptococcus species.
- 10 mg streptomycin per mL: Streptomycin is an antibiotic that targets Gram-negative bacteria and mycoplasma. It complements the action of penicillin by inhibiting the growth of additional bacterial contaminants.
- 0.9% NaCl: Sodium chloride (NaCl) is commonly used as a solution to maintain osmotic balance and prevent cell shrinkage in cell culture media.
Usage:
The 10,000U Penicillin 10mg/mL Streptomycin 200 mM L-Gln L-Glutamine–Penicillin–Streptomycin Solution is primarily intended for use in mammalian cell culture to eliminate bacterial contamination. It is particularly suitable for primary cell cultures, where maintaining a pure and uncontaminated culture is crucial.
Instructions for usage:
- Ensure that the solution is sterile and undamaged before use.
- Thaw the solution if frozen and bring it to room temperature.
- Add the desired volume of the solution to the cell culture media at the recommended concentration (usually 1% to 10% v/v).
- Mix gently to ensure uniform distribution of the solution throughout the media.
- Proceed with the intended cell culture protocols.
Storage:
The 10,000U Penicillin 10mg/mL Streptomycin 200 mM L-Gln L-Glutamine–Penicillin–Streptomycin Solution should be stored in a refrigerator at 2-8°C. It is recommended to protect the solution from light and avoid repeated freeze-thaw cycles. When stored properly, the solution has a shelf life of approximately 12 months.
Precautions:
- For research use only. Not for use in diagnostic or therapeutic procedures.
- Avoid direct contact with skin, eyes, and clothing. In case of contact, rinse thoroughly with water.
- Handle the solution with appropriate safety measures to avoid ingestion or inhalation.
- Dispose of the solution and its container in accordance with local regulations and guidelines.
Conclusion:
The 10,000U Penicillin 10mg/mL Streptomycin 200 mM L-Gln L-Glutamine–Penicillin–Streptomycin Solution is a reliable and effective option for reducing or eliminating bacterial contamination in mammalian cell cultures. Its composition of L-glutamine, penicillin, streptomycin, and sodium chloride ensures optimal conditions for cell growth while maintaining the purity and quality of the culture. With proper storage and usage, this solution provides researchers with a valuable tool to achieve successful and uncontaminated cell cultures.
